株式会社エイエルエイ中央研究所

エイエルエイ中央研究所トップ学会発表2017年>第59回歯科基礎医学会学術大会

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第59回歯科基礎医学会学術大会

Determination of the antibacterial substances produced by probiotic lactobacilli against a periodontal pathogen

Tomomi Kawai1)

Tomoko Ohshima1) Ryoichi Shin2) Satoshi Ikawa3) Atsushi Tani4) Naoya Inazumi5) Nobuko Maeda1)
1)Dept. Oral Microbiol, Sch. Dental Med., Tsurumi Univ.
2)Central Institute, Health Science, A.L.A. Corporation
3)Technology Research Institute, Osaka Prefecture
4)Dept. Human Environmental Science, Faculty of Human Development, Kobe Univ.
5)Technical Support Division, Graduate Sch. Science, Osaka Univ.

【Objectives】

Probiotics are living bacteria which can improve the balance of microbiota. There are many recent studies on the effects of probiotics, including oral health promotion and the prevention of oral diseases. However, the mechanisms that underlie the activity of probiotic bacteria against periodontal pathogens have not been clearly elucidated. In order to clarify the antibacterial mechanisms of probiotics against periodontal pathogens, we have performed to identify antibacterial substances produced by Lactobacillus fermentum ALAL020 with the highest antibacterial activity against Porphyromonas gingivalis among the screened 50 strains of lactobacilli.

【Methods】

The culture supernatant in MRS broth was neutralized and purified by gel filtration column chromatography and reverse-phase HPLC. The molecular weight and structure of purified substances were analyzed with LC-MS and NMR.

【Results】

The major antibacterial substance in culture supernatant produced by L. fermentum ALAL020 was purified as a single peak fraction and the molecular weight (m/z) was 226.131. An LC-MS analysis of this low molecular weight compound revealed that the composition formula was C11H18O3N2. The results of structural analysis by NMR showed as a cyclic di-peptide, hexahydro-7-hydroxy-3-(2-methylproryl)pyrrolo[1,2-a]pyrazine-1,4-dione.

【Conclusions】

We purified and determined an antibacterial substance produced by L. fermentum ALAL020 which was main active constituent against P. gingivalis. This substance was suggested as a novel substance produced by a probiotic bacterium, and might have a possibility for prevention of periodontal disease.